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G8142

Sigma

 

Grace’s Insect Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, insect cell culture tested

MDL number:MFCD00147059

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Description

ApplicationGrace's medium was originally formulated to support the growth of cells derived from the Australian emperor gum moth, Antherea eucalypti. The medium is a modification of Wyatt's medium to more closely resemble Antherea hemolymph. The cell lines established by Grace using this medium were the first continuous lines developed. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of lepidopterans as well as some dipterans. The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans.

Properties

sterilitysterile-filtered
formliquid
suitabilityinsect cell culture tested
storage temp.2-8°C

Safety

WGK Germany3

References

referenceGrace, T.D.C., Establishment of four strains of cells from insect tissues grown in vitro. Nature 195, 788, (1962)
 Grace, T.D.C., Establishment of a line of cells from the silkworm Bombyx mori.,. Nature 216, 613, (1967)
 Grace, T.D.C., Establishment of a line of mosquito (Aedes aegypti) cells grown in vitro. Nature 211, 366, (1966)